Skip to main content

Hydrosensing Project

Discovering How Plants Sense Water Stress

The Hydrosensing Project Team is on a mission to transform our understandingof how plants sense and respond to water availability. We aim to uncover the mechanisms plants use to perceive water stress, a key factor in their survival and productivity.

HYDROSENSING

a Synergy Project

By combining cutting-edge genomics, structural biology, biophysics and imaging approaches, we strive to revolutionize crop resilience and pave the way for climate-proof agriculture.

Join us as we explore new frontiers in plant science, working towards a future where crops are better equipped to withstand the challenges of a changing climate.

Publications

Journal articles and preprints by the Hydrosensing project

Schematic overview illustrating the library workflow, from design to screening. All coding genes were divided into phylogenetic trees, and trees were classified by function. sgRNAs were designed to target multiple genes located in close proximity to one another phylogenetically (indicated by colors). Each sgRNA was cloned into the Cas9 vector, creating a plasmid library. Transformed lines were screened with multi-targeted, large-scale, forward genetics for specific traits of interest, revealing hidden phenotypes. *Whole genome indicates all coding genes excluding transporters, transcription factors, and enzymes.
Publications

Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level

Genetic variation drives crop breeding, but traditional mutagenesis is limited by gene redundancy and low…
https://doi.org/10.1038/s41586-025-08941-z
Publications

Single-cell transcriptomics reveal how root tissues adapt to soil stress

Scientists have discovered, for the first time how root cells respond to their complex soil…
Double knockdown of ABCG17 and ABCG18 leads to changes in ABA response in the valves. A, pRAB18:GFP signal in mir17,g18 and Control siliques at 4 DAP. mir17,g18 is mir17 (amiRNA-ABCG17) transformed into the background of abcg18-1 T-DNA insertion line. Shown are two independent transformation lines (#1 and #5). Green is GFP fluorescent signal, and red is chlorophyll. Scale bar = 50 m. B, Average (±SD) GFP intensities in indicated lines. n ≥ 4; *, P value < 0.05, **, P value < 0.01, Student’s t test
Publications

ABA importers ABCG17 and ABCG18 redundantly regulate seed size in Arabidopsis

The stress hormone abscisic acid (ABA) plays a crucial role in mediating plant responses to…
Dynamic interactions at the root–soil interface.(e) high-resolution X-ray computed tomography images reveal how plant roots impact the structural development of the rhizosphere by causing changes in soil pore thickness over time. Panel e adapted from Reference 48 (CC BY 4.0).
Publications

Root Growth and Development in “Real Life”: Advances and Challenges in Studying Root–Environment Interactions

Plant roots play myriad roles that include foraging for resources in complex soil environments. Within…