Skip to main content
Targeting redundant gene families: A multiplexed,tissue-specific CRISPR toolbox for Arabidopsisgenetic screens Publications
March 12, 2026

Targeting redundant gene families: A multiplexed,tissue-specific CRISPR toolbox for Arabidopsisgenetic screens

Anfang M, Yahya RH, Caldararu O, Ben Yaakov S, Landau U, Berman A, Hu Y, Belew ZM, Crocoll C, Xu D, Nour-Eldin HH, Mayrose I, Shani E. Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens. Cell Rep. 2026 Mar 9;45(3):117055. doi: 10.1016/j.celrep.2026.117055.
Hydrosensing
Image by Gundula Vogel from Pixabay
Developmental pathways in plants: Lessons from Arabidopsis for crop innovation  Publications
January 27, 2026

Developmental pathways in plants: Lessons from Arabidopsis for crop innovation 

Malcolm J Bennett, Rahul Bhosale, Scott A Boden, Shu-Yan Chen, Tino Colombi, Toshiro Ito, Hong-Ju Li, Poonam Mehra, Lars Østergaard, Meng Li, Liu Liu, Nana Otsuka, Bipin K Pandey, Scott Poethig, Kalika Prasad, Yue Qu, Makoto Shirakawa, Ying Hua Su, Cao Xu, Wei-Cai Yang, Wen Jie Zhang, Xiaolan Zhang, Xian…
Hydrosensing
Image: Description and validation of pMDS plasmid system for dual analysis of transcription and translation in plants. (A) Organization of pMDS1 vector showing reporters for transcription [mTurquoise (mTurQ)], translation (C-terminal mVenus), and a 2A self-cleaving peptide. (B) Organization of pMDS2 vector showing reporters for transcription (mTurQ), translation (N-terminal mVenus), and a 2A self-cleaving peptide. (C) Confocal image of pMDS1_SHRpro:SHR:mVenus:mTurQ showing gene expression (mTurQ) in the stele region and protein (mVenus) translocating to endodermis in the root meristem. (D) Confocal image of pMDS2_VAM3pro:mVenus:VAM3:mTurQ showing subcellular expression (mTurQ) in the nucleus and protein (mVenus) moving to the vacuole in the root epidermis. Red channel shows mCherry expression. nu, nucleus; vac, vacuole; *, endodermis of root meristem. Scale bar, 10 μM. Credit: Science Advances
Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote Publications
January 21, 2026

Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote

Jason Banda et al. Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote Science Advances 11,eadw9153(2025). DOI:10.1126/sciadv.adw9153
Hydrosensing
Fig. 1. Expression pattern of cell type-specific promoters used to drive multi-targeted amiRNA libraries. A) Shown are confocal or stereoscope images for pML1:H2B-GFP (shoot epidermis), pIQD22:GUS-mCitrine (palisade mesophyll), pSCR:YFP (endodermis and bundle sheath), pSUC2:YFP (phloem companion cells), pCORI3:GUS-mCitrine (spongy mesophyll), pKST1:GFP (guard cells), pS18:YFP (xylem), pPGP4:NLS-YFP (root epidermis and cortex), pCO2:YFP (cortex), pUBQ10:YFP (constitutive), pSIG6:GUS (shoot), and pARSK1:GUS (root). Red signal indicates cell wall dye propidium iodide. Blue signal in pIQD22 and pCORI3 indicates chlorophyll auto-fluorescence. pML1, pSCR, pSUC2, pKST1, pS18, pPGP4, pCO2, and pUBQ10 seedlings are 5 days old, pSIG6 and pARSK1 seedlings are 12 days old, and pIQD22 and pCORI3 are 17 days old. Scale bars = 50 µm for pML1, pIQD22, pSCR, pSUC2, pCORI3, pKST1, pS18, pPGP4, pCO2, and pUBQ10; scale bar = 1 mm for pARSK1; and scale bar = 2 mm for pSIG6. B) Illustrations of the cell type-specific expression patterns driven by promoters used in this study to drive multi-targeted amiRNA expression. Illustrations are of leaf tissue (upper left), root cross-section (lower left), root tip (center) and whole plant (right).
mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis  Publications
January 20, 2026

mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis 

Moran Anfang, Shir Ben Yaakov, Ning Su, Anat Shafir, Jenia Binenbaum, Reem Haj Yahya, Xikai Yu, Carl Procko, Hamtual Bar, Joanne Chory, Julian I Schroeder, Yosef Fichman, Itay Mayrose, Eilon Shani, Yuqin Zhang, mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis, Plant Physiology, 2025;, kiaf682, https://doi.org/10.1093/plphys/kiaf682
Hydrosensing
Dynamic interactions at the root–soil interface.(e) high-resolution X-ray computed tomography images reveal how plant roots impact the structural development of the rhizosphere by causing changes in soil pore thickness over time. Panel e adapted from Reference 48 (CC BY 4.0).
Root Growth and Development in “Real Life”: Advances and Challenges in Studying Root–Environment Interactions Publications
March 20, 2025

Root Growth and Development in “Real Life”: Advances and Challenges in Studying Root–Environment Interactions

Mehra, P., Banda, J., Ogorek, L. L. P., Fusi, R., Castrillo, G., Colombi, T., ... & Bennett, M. J. (2025). Root Growth and Development in “Real Life”: Advances and Challenges in Studying Root–Environment Interactions. Annual Review of Plant Biology, 76. https://doi.org/10.1146/annurev-arplant-083123-074506
Hydrosensing
Fig. 1. Hydropatterning responses revealed in public sector breeding lines of Zea mays (maize)
Moisture-responsive root-branching pathways identified in diverse maize breeding germplasm Publications
February 24, 2025

Moisture-responsive root-branching pathways identified in diverse maize breeding germplasm

Scharwies, J. D., Clarke, T., Zheng, Z., Dinneny, A., Birkeland, S., Veltman, M. A., … & Dinneny, J. R. (2025). Moisture-responsive root-branching pathways identified in diverse maize breeding germplasm. Science, 387(6734), 666-673. https://www.science.org/doi/abs/10.1126/science.ads5999 .
Hydrosensing