Publications – ERC SYNERGY Project HYDROSENSING

Mar 17

Image: Mixed crystalloid-ER and whorl morphology. Central slice through a STEM tomogram showing a patch of crystalloid-ER (dark blue) located in close proximity to a putative ER whorl (light blue) in its intracellular environment. Closer inspection of the reconstructed tomogram reveals a direct connection between the two ER morphotypes (I), as well as the presence of multiple vesicles (not present in the central tomogram section), located near the nucleus (orange) and contacting the crystalloid ER (II). Arrowheads indicate the viewing direction in Figure 6. Scale bar = 500 nm. Credit: Journal of Microscopy

Application of STEM tomography to investigate smooth ER morphology under stress conditions

By Hydrosensing Publications

Heinz, V., Rachel, R., & Ziegler, C. (2025). Application of STEM tomography to investigate smooth ER morphology under stress conditions. Journal of Microscopy, 299(3), 228-241. https://doi.org/10.1111/jmi.70020

Mar 12

Targeting redundant gene families: A multiplexed,tissue-specific CRISPR toolbox for Arabidopsisgenetic screens

By Hydrosensing Publications

Anfang M, Yahya RH, Caldararu O, Ben Yaakov S, Landau U, Berman A, Hu Y, Belew ZM, Crocoll C, Xu D, Nour-Eldin HH, Mayrose I, Shani E. Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens. Cell Rep. 2026 Mar 9;45(3):117055. doi: 10.1016/j.celrep.2026.117055.

Feb 19

The mechanical properties of Arabidopsis thaliana roots adapt dynamically during development and to stress

By Hydrosensing Publications

Luis Alonso Baez et al., The mechanical properties of Arabidopsis thaliana roots adapt dynamically during development and to stress. Sci. Adv. 12,eaeb0032(2026).DOI:10.1126/sciadv.aeb0032

Feb 12

Transgenerational polarity axis inheritance during Ceratopterisembryogenesis

By Hydrosensing Publications

Woudenberg, S., Plackett, A. R., Hao, Z., Suzuki, H., Baez, L. A., Borassi, C., ... & Weijers, D. (2025). Transgenerational polarity axis inheritance during Ceratopteris embryogenesis. bioRxiv, 2025-08 https://doi.org/10.1101/2025.08.29.673061

Feb 02

a, Semi-thin sections (2 µm thickness, 1 cm from root tip) of WT, arf1-1, OE-ARF1, cesa6 and arf1cesa6 lines. Scale bar=50 µm.

Ethylene modulates cell wall mechanics for root responses to compaction

By Hydrosensing Publications

Zhang, J., Liu, Z., Farrar, E.J. et al. Ethylene modulates cell wall mechanics for root responses to compaction. Nature 649, 685–692 (2026). https://doi.org/10.1038/s41586-025-09765-7

Jan 27

Developmental pathways in plants: Lessons from Arabidopsis for crop innovation

By Hydrosensing Publications

Malcolm J Bennett, Rahul Bhosale, Scott A Boden, Shu-Yan Chen, Tino Colombi, Toshiro Ito, Hong-Ju Li, Poonam Mehra, Lars Østergaard, Meng Li, Liu Liu, Nana Otsuka, Bipin K Pandey, Scott Poethig, Kalika Prasad, Yue Qu, Makoto Shirakawa, Ying Hua Su, Cao Xu, Wei-Cai Yang, Wen Jie Zhang, Xiaolan Zhang, Xian…

Jan 21

Image: Description and validation of pMDS plasmid system for dual analysis of transcription and translation in plants. (A) Organization of pMDS1 vector showing reporters for transcription [mTurquoise (mTurQ)], translation (C-terminal mVenus), and a 2A self-cleaving peptide. (B) Organization of pMDS2 vector showing reporters for transcription (mTurQ), translation (N-terminal mVenus), and a 2A self-cleaving peptide. (C) Confocal image of pMDS1_SHRpro:SHR:mVenus:mTurQ showing gene expression (mTurQ) in the stele region and protein (mVenus) translocating to endodermis in the root meristem. (D) Confocal image of pMDS2_VAM3pro:mVenus:VAM3:mTurQ showing subcellular expression (mTurQ) in the nucleus and protein (mVenus) moving to the vacuole in the root epidermis. Red channel shows mCherry expression. nu, nucleus; vac, vacuole; *, endodermis of root meristem. Scale bar, 10 μM. Credit: Science Advances

Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote

By Hydrosensing Publications

Jason Banda et al. Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote Science Advances 11,eadw9153(2025). DOI:10.1126/sciadv.adw9153

Jan 20

Fig. 1. Expression pattern of cell type-specific promoters used to drive multi-targeted amiRNA libraries. A) Shown are confocal or stereoscope images for pML1:H2B-GFP (shoot epidermis), pIQD22:GUS-mCitrine (palisade mesophyll), pSCR:YFP (endodermis and bundle sheath), pSUC2:YFP (phloem companion cells), pCORI3:GUS-mCitrine (spongy mesophyll), pKST1:GFP (guard cells), pS18:YFP (xylem), pPGP4:NLS-YFP (root epidermis and cortex), pCO2:YFP (cortex), pUBQ10:YFP (constitutive), pSIG6:GUS (shoot), and pARSK1:GUS (root). Red signal indicates cell wall dye propidium iodide. Blue signal in pIQD22 and pCORI3 indicates chlorophyll auto-fluorescence. pML1, pSCR, pSUC2, pKST1, pS18, pPGP4, pCO2, and pUBQ10 seedlings are 5 days old, pSIG6 and pARSK1 seedlings are 12 days old, and pIQD22 and pCORI3 are 17 days old. Scale bars = 50 µm for pML1, pIQD22, pSCR, pSUC2, pCORI3, pKST1, pS18, pPGP4, pCO2, and pUBQ10; scale bar = 1 mm for pARSK1; and scale bar = 2 mm for pSIG6. B) Illustrations of the cell type-specific expression patterns driven by promoters used in this study to drive multi-targeted amiRNA expression. Illustrations are of leaf tissue (upper left), root cross-section (lower left), root tip (center) and whole plant (right).

mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis

By Hydrosensing Publications

Moran Anfang, Shir Ben Yaakov, Ning Su, Anat Shafir, Jenia Binenbaum, Reem Haj Yahya, Xikai Yu, Carl Procko, Hamtual Bar, Joanne Chory, Julian I Schroeder, Yosef Fichman, Itay Mayrose, Eilon Shani, Yuqin Zhang, mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis, Plant Physiology, 2025;, kiaf682, https://doi.org/10.1093/plphys/kiaf682

Nov 03

Image: Short-day Arabidopsis plants experiment for drought and ABA2 content. Credit: Barak Bitman, Tel Aviv University

In defense of funding foundational plant science

By Hydrosensing Publications

Joanna D Friesner, Cristiana T Argueso, Wolfgang Busch, Thorsten Hamann, Lucia Strader, Mary Williams, Shuang Wu, Adrienne H K Roeder, In defense of funding foundational plant science, The Plant Cell, Volume 37, Issue 5, May 2025, koaf106, https://doi.org/10.1093/plcell/koaf106

Aug 31

Image: Thermal imaging of tomato. Credit: Barak Bitman, Tel Aviv University

Arabidopsis research in 2030: Translating the computable plant

By Hydrosensing Publications

Brady, S., Auge, G., Ayalew, M., Balasubramanian, S., Hamann, T., Inze, D., ... & Provart, N. J. (2025). Arabidopsis research in 2030: Translating the computable plant. The Plant Journal, 121(5), e70047.