Publications – Page 2 – ERC SYNERGY Project HYDROSENSING

Jan 21

Image: Description and validation of pMDS plasmid system for dual analysis of transcription and translation in plants. (A) Organization of pMDS1 vector showing reporters for transcription [mTurquoise (mTurQ)], translation (C-terminal mVenus), and a 2A self-cleaving peptide. (B) Organization of pMDS2 vector showing reporters for transcription (mTurQ), translation (N-terminal mVenus), and a 2A self-cleaving peptide. (C) Confocal image of pMDS1_SHRpro:SHR:mVenus:mTurQ showing gene expression (mTurQ) in the stele region and protein (mVenus) translocating to endodermis in the root meristem. (D) Confocal image of pMDS2_VAM3pro:mVenus:VAM3:mTurQ showing subcellular expression (mTurQ) in the nucleus and protein (mVenus) moving to the vacuole in the root epidermis. Red channel shows mCherry expression. nu, nucleus; vac, vacuole; *, endodermis of root meristem. Scale bar, 10 μM. Credit: Science Advances

Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote

By Hydrosensing Publications

Jason Banda et al. Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote Science Advances 11,eadw9153(2025). DOI:10.1126/sciadv.adw9153

Jan 20

Fig. 1. Expression pattern of cell type-specific promoters used to drive multi-targeted amiRNA libraries. A) Shown are confocal or stereoscope images for pML1:H2B-GFP (shoot epidermis), pIQD22:GUS-mCitrine (palisade mesophyll), pSCR:YFP (endodermis and bundle sheath), pSUC2:YFP (phloem companion cells), pCORI3:GUS-mCitrine (spongy mesophyll), pKST1:GFP (guard cells), pS18:YFP (xylem), pPGP4:NLS-YFP (root epidermis and cortex), pCO2:YFP (cortex), pUBQ10:YFP (constitutive), pSIG6:GUS (shoot), and pARSK1:GUS (root). Red signal indicates cell wall dye propidium iodide. Blue signal in pIQD22 and pCORI3 indicates chlorophyll auto-fluorescence. pML1, pSCR, pSUC2, pKST1, pS18, pPGP4, pCO2, and pUBQ10 seedlings are 5 days old, pSIG6 and pARSK1 seedlings are 12 days old, and pIQD22 and pCORI3 are 17 days old. Scale bars = 50 µm for pML1, pIQD22, pSCR, pSUC2, pCORI3, pKST1, pS18, pPGP4, pCO2, and pUBQ10; scale bar = 1 mm for pARSK1; and scale bar = 2 mm for pSIG6. B) Illustrations of the cell type-specific expression patterns driven by promoters used in this study to drive multi-targeted amiRNA expression. Illustrations are of leaf tissue (upper left), root cross-section (lower left), root tip (center) and whole plant (right).

mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis

By Hydrosensing Publications

Moran Anfang, Shir Ben Yaakov, Ning Su, Anat Shafir, Jenia Binenbaum, Reem Haj Yahya, Xikai Yu, Carl Procko, Hamtual Bar, Joanne Chory, Julian I Schroeder, Yosef Fichman, Itay Mayrose, Eilon Shani, Yuqin Zhang, mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis, Plant Physiology, 2025;, kiaf682, https://doi.org/10.1093/plphys/kiaf682

Nov 03

Image: Short-day Arabidopsis plants experiment for drought and ABA2 content. Credit: Barak Bitman, Tel Aviv University

In defense of funding foundational plant science

By Hydrosensing Publications

Joanna D Friesner, Cristiana T Argueso, Wolfgang Busch, Thorsten Hamann, Lucia Strader, Mary Williams, Shuang Wu, Adrienne H K Roeder, In defense of funding foundational plant science, The Plant Cell, Volume 37, Issue 5, May 2025, koaf106, https://doi.org/10.1093/plcell/koaf106

Aug 31

Image: Thermal imaging of tomato. Credit: Barak Bitman, Tel Aviv University

Arabidopsis research in 2030: Translating the computable plant

By Hydrosensing Publications

Brady, S., Auge, G., Ayalew, M., Balasubramanian, S., Hamann, T., Inze, D., ... & Provart, N. J. (2025). Arabidopsis research in 2030: Translating the computable plant. The Plant Journal, 121(5), e70047.

Jul 17

Consensus statement on Brillouin light scattering microscopy of biological materials

By Hydrosensing Publications

Bouvet, P., Bevilacqua, C., Ambekar, Y., Antonacci, G., Au, J., Caponi, S., ... & Elsayad, K. (2025). Consensus statement on Brillouin light scattering microscopy of biological materials. Nature Photonics, 19(7), 681-691. https://doi.org/10.1038/s41566-025-01681-6

Jun 16

Redox-regulated Aux/IAA multimerization modulates auxin responses

By Hydrosensing Publications

Roy, D., Mehra, P., Clark, L., Mukkawar, V., Bellande, K., Martin-Arevalillo, R., ... & Sadanandom, A. (2025). Redox-regulated Aux/IAA multimerization modulates auxin responses. Science, eadu1470. https://www.science.org/doi/10.1126/science.adu1470

Jun 06

Future crop breeding needs to consider future soils

By Hydrosensing Publications

Raza, S., Pandey, B.K., Hawkesford, M.J. et al. Future crop breeding needs to consider future soils. Nature Plants 11, 939–941 (2025). https://www.nature.com/articles/s41477-025-01977-z

May 05

Schematic overview illustrating the library workflow, from design to screening. All coding genes were divided into phylogenetic trees, and trees were classified by function. sgRNAs were designed to target multiple genes located in close proximity to one another phylogenetically (indicated by colors). Each sgRNA was cloned into the Cas9 vector, creating a plasmid library. Transformed lines were screened with multi-targeted, large-scale, forward genetics for specific traits of interest, revealing hidden phenotypes. *Whole genome indicates all coding genes excluding transporters, transcription factors, and enzymes.

Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level

By Hydrosensing Publications

Berman, A., Su, N., Li, Z. et al. Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level. Nat Commun 16, 4111 (2025). https://doi.org/10.1038/s41467-025-59280-6

May 02

https://doi.org/10.1038/s41586-025-08941-z

Single-cell transcriptomics reveal how root tissues adapt to soil stress

By Hydrosensing Publications

Zhu, M., Hsu, C. W., Peralta Ogorek, L. L., Taylor, I. W., La Cavera, S., Oliveira, D. M., ... & Pandey, B. K. (2025). Single-cell transcriptomics reveal how root tissues adapt to soil stress. Nature, 1-9. https://doi.org/10.1038/s41586-025-08941-z

Mar 21

Double knockdown of ABCG17 and ABCG18 leads to changes in ABA response in the valves. A, pRAB18:GFP signal in mir17,g18 and Control siliques at 4 DAP. mir17,g18 is mir17 (amiRNA-ABCG17) transformed into the background of abcg18-1 T-DNA insertion line. Shown are two independent transformation lines (#1 and #5). Green is GFP fluorescent signal, and red is chlorophyll. Scale bar = 50 m. B, Average (±SD) GFP intensities in indicated lines. n ≥ 4; *, P value < 0.05, **, P value < 0.01, Student’s t test

ABA importers ABCG17 and ABCG18 redundantly regulate seed size in Arabidopsis

By Hydrosensing Publications

Zhang, Y., Anfang, M., Rowe, J.H., Rizza, A., Li, Z., Su, N., Bar, H., Charrier, L., Geisler, M., Jones, A.M. and Shani, E. (2025), ABA importers ABCG17 and ABCG18 redundantly regulate seed size in Arabidopsis. Plant J, 121: e70096. https://doi.org/10.1111/tpj.70096